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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Bone. 2019 Jul 17;127:488–498. doi: 10.1016/j.bone.2019.07.017

Figure 1: Overview of CRISPR strategy and outcomes.

Figure 1:

A) Diagram of Satb2 protein with the location of the Cas9 cut site, qPCR primer sets, and the binding region of the antibody used in this study highlighted. B) Colony cell lines used in this study are described. The colony number, Satb2 genotype, cut site DNA sequence and translation, as well as protein product produced are listed.