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. 2019 May 23;65(4):353–359. doi: 10.1262/jrd.2019-058

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©2019 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 1. — Preservation of freeze-dried (FD) sperm for up to 3 months at RT and production of offspring. (a, b) Ampoules of FD sperm in plastic bags were preserved in a desk drawer. (c) Blastocysts derived from oocytes fertilized with FD sperm treated with 0.1 to 2.0 M trehalose or without trehalose and preserved at RT for 1 month. (d) Healthy offspring derived from FD sperm preserved at RT for 1 month with 0.5 M trehalose. (e) Birth rates of embryos derived from the FD sperm of inbred strains. Those FD sperm were stored with or without 0.5 M trehalose and were preserved for 3 months at RT. Asterisk indicates the significant difference between samples treated and non-treated with trehalose in each strain (P < 0.01).