Table 2. The rate of blastocyst development and full-term embryonic development after fertilization with freeze-dried sperm preserved for 1 week.
| Conc. of trehalose | No. of oocytes surviving after ICSI |
No. (%) of fertilized zygotes |
No. (%) of embryos developed to |
No. of transferred embryos (no. of recipients) |
No. (%) [min-max] of offspring | Mean body weight (g) | |||
|---|---|---|---|---|---|---|---|---|---|
| 2 cell (%) | 4–8 cell (%) | Morula (%) | Blastocyst (%) | ||||||
| 0 M | 47 | 46 (98) | 43 (93) | 33 (72) | 28 (61) | 19 (41) | – | – | – |
| 35 | 35 (100) | 27 (77) | – | – | – | 27 (3) | 4 (15) [9–20] | 2.06 ± 0.21 | |
| 0.1 M | 36 | 35 (97) | 32 (91) | 23 (66) | 23 (66) | 15 (43) | – | – | – |
| 35 | 35 (100) | 35 (100) | – | – | – | 35 (3) | 6 (17) [0–33] | 2.08 ± 0.07 | |
| 0.5 M | 52 | 49 (94) | 43 (88) | 39 (80) | 36 (73) | 26 (53) | – | – | – |
| 46 | 46 (100) | 40 (87) | – | – | – | 40 (3) | 14 (35) [30–7] | 1.85 ± 0.17 | |
| 2.0 M | 41 | 36 (88) | 29 (81) | 22 (61) | 20 (56) | 13 (36) | – | – | – |
| 63 | 61 (97) | 50 (82) | – | – | – | 34 (4) | 5 (15) [0–40] | 2.09 ± 0.14 | |