Table 3. The rate of blastocyst development and full-term embryonic development after fertilization with freeze-dried sperm preserved for 1 month.
| Conc. of trehalose | No. of oocytes surviving after ICSI |
No. (%) of fertilized zygotes |
No. (%) of embryos developed to |
No. of transferred embryos (no. of recipients) |
No. (%) [min–max]* of offspring | Mean body weight (g) | |||
|---|---|---|---|---|---|---|---|---|---|
| 2 cell (%) | 4–8 cell (%) | Morula (%) | Blastocyst (%)* | ||||||
| 0 M | 57 | 55 (96) | 45 (82) | 30 (55) | 23 (42) | 16 (29) a | – | – | – |
| 91 | 88 (97) | 69 (78) | – | – | – | 60 (5) | 13 (22) [0–75] | 1.76 ± 0.32 | |
| 0.1 M | 46 | 40 (87) | 35 (88) | 30 (75) | 26 (65) | 25 (63) b | – | – | – |
| 56 | 51 (91) | 45 (88) | – | – | – | 44 (4) | 12 (27) [20–38] | 1.83 ± 0.12 | |
| 0.5 M | 96 | 90 (94) | 76 (84) | 61 (68) | 61 (68) | 50 (56) b | – | – | – |
| 41 | 38 (93) | 33 (87) | – | – | – | 32 (3) | 15 (47) [25–56] a | 1.93 ± 0.14 | |
| 2.0 M | 40 | 37 (93) | 29 (78) | 25 (68) | 20 (54) | 14 (38) | – | – | – |
| 64 | 58 (91) | 52 (90) | – | – | – | 46 (5) | 8 (17) [0–50] b | 1.94 ± 0.14 | |
* Different letters mean significant differences (P < 0.01).