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Methylation changes as revealed by COBRA at three DMRs (A: CpG-1, B: CpG-A1, C: CpG-A5) in MII oocytes before IVF (white circles, pooled n = 70), spermatozoa (white bars, JD2–5), and IVF-derived blastocyst embryos (black circles, n = 5–17, analyzed one by one) using JD2–5 spermatozoa. Bars indicate average methylation levels.
