Table 3. IVF results of frozen semen samples of the same Japanese Black bull at different ages (JD1~5).
| Semen | Age | Motility | IVF |
||||
|---|---|---|---|---|---|---|---|
| (months) | Total (r) | Cleaved (%) | Blastocyst (%) | ||||
| JD1 | 14.0 | 5 | 86 (2) | 7 | (8.1) a | 0 | (0.0) a |
| JD2 | 19.3 | 50 | 141 (3) | 87 | (61.7) bc | 24 | (33.3) b |
| JD3 | 28.1 | 43 | 122 (3) | 91 | (72.8) b | 23 | (33.6) b |
| JD4 | 54.2 | 37 | 158 (3) | 88 | (55.7) c | 25 | (29.1) b |
| JD5 | 162.0 | 57 | 133 (3) | 70 | (52.6) c | 15 | (24.1) b |
Motility: determined under microscope just after thawing. Total: number of oocytes cultured after in vitro fertilization. r: repeat number for IVF experiment. a ,b, c The values with different superscripts within the same column are significantly different (P < 0.05).