Figure 5.

Further analysis of FRET ratiometric images. A and B. Example FRET ratiometric images of the cell in Fig. 4 taken at 0 s (A) and 900 s (B) from the stimulation. The white box in both images refers to the area used in the image montage in (C). Scale bar in Fig. 5B refers to Fig. 5A and 5B: 20 µm. C. Image montage of the region highlighted by the white box in (A) and (B). Images are taken every 60 s. The intensity scale on the right refers to Fig. 5A, 5B and 5C. D. Plot of the RhoA activity, normalized by the initial ratio value (ΔR/R₀), in the edge region highlighted by the black square in (C) vs. edge retraction that shows the increase in RhoA activity as the growth cone retracts.