Fig. 1.

Average frequency of isotype 1 β-tubulin locus SNPs using four different PCR cycles for six T. circumcincta laboratory populations. This data was generated from pooled samples with about 200 L₃. The F200Y (TAC) resistance alleles were identified in all six populations with different frequencies. The F167Y (TAC) was found only in the 6-R population (Supplementary Table S3). Dark grey shade indicates F167Y (TAC), medium grey indicates F200Y (TAC) and white indicates susceptible alleles. X-axis represents the four PCR cycles numbers (25X, 30X, 35X, 40X) and Y-axis represents the allele frequencies. Error bars represent the standard error of the mean.