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Average frequency of the isotype 1 β-tubulin locus F200Y (TAC) SNP in mock pools was made from different mixing of pyrosequence genotyped 1-S individual homozygous susceptible and 5-R homozygous resistant T. circumcincta L3. The symbol mix represents the mixing of resistant-R and susceptible-S alleles. In the X-axis, E represents the expected allele frequencies and O represents the observed allele frequencies based on Illumina MiSeq.
