Figure 1.

miR-596 targets Survivin’s 3′-UTR. (A) The bold, italicized type image indicates the potential miR-596-binding sites or mutated sites located in Survivin’s 3′-UTR. (B) U2OS cells, which were transfected with control (control miRNA), miR-596, miR-596 + Survivin^Mut, or miR-596c + its inhibitor, were harvested for Western blot experiments. The protein level of Survivin or GAPDH in U2OS cells was examined by their antibodies, and GAPDH was chosen as the loading control. Results were shown as images from Western blot (B) or quantitative analysis results (C). *P<0.05.

Abbreviations: miR, microRNA; Mut, mutation.