Figure 6.

Calcium transients evoked by nerve stimulation at various frequencies. A, Example transients from one cell following stimulation for 0.5 s at frequencies from 1 to 300 Hz. For reference, the standard stimulation of 25 action potentials used elsewhere in this study is shown in yellow. B, Resting fluorescence measured in arbitrary units does not change with increasing stimulation frequencies. C–F, Plots of transient parameter changes with varying stimulation frequencies. Error bars indicate SEM for responses evoked by stimulation frequencies of 10 Hz and higher.