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. 2019 Aug 1;6(4):ENEURO.0425-18.2019. doi: 10.1523/ENEURO.0425-18.2019

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Copyright © 2019 Nanguneri et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Analysis of nano-organization of F-actin at 1-nm/px sampling. A, PREM image of cytoskeletal distribution within a spine. Scale bar = 200 nm. B, The segmented image selecting only the thin filaments in PREM indicate the F-actin distribution. C, ANNA-PALM simulation of the F-actin network using tubular model. D, Extraction of ridges by skeletonizing the ANNA-PALM image. E, Overlay of an image obtained by PREM (green) and ridges that mark the F-actin network (red) of the spine. Scale bar = 200 nm. F, G, Magnified views of sections within the spine; please refer to Extended Data Fig. 3-1 for a comparative analysis between PREM and simulated super resolved images. The ridges overlapped with the PREM images with a correlation of >89%. Scale bar = 50 nm.