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. 2019 Aug 5;6(4):ENEURO.0492-18.2019. doi: 10.1523/ENEURO.0492-18.2019

Figure 1.

Figure 1.

(Gi)DREADD is expressed in V2a neurons in the spinal cord and brainstem of V2a-(Gi)DREADD mice. Immunohistochemistry using antibodies to Chx10 and the HA tag on (Gi)DREADD were used to label the nuclei of V2a neurons and (Gi)DREADD expressing cells, respectively, in V2a-(Gi)DREADD mice. A, C4 cervical spinal cord section (spinal cord boundary outlined) of a neonatal (P2) mouse showing (Gi)DREADD expression in the intermediate lamina where V2a neurons are found. Scale bar = 100 μm. B, Coronal section of the medulla of a neonatal (P2) mouse at the level of the nucleus ambiguous showing (Gi)DREADD expression in the medial reticular formation outlined (dotted line) where V2a neurons are found. Scale bar = 200 μm. C, C4 cervical spinal cord section (gray matter outlined) of an adult mouse showing (Gi)DREADD expression in the intermediate lamina where V2a neurons are found. Scale bar = 100 μm. D, Coronal section of the medulla of an adult mouse at the level of the nucleus ambiguous showing (Gi)DREADD expression in the medial reticular formation outlined (dotted line) where V2a neurons are found. Scale bar = 200 μm. Insets, Boxed regions in A–D. (Gi)DREADD+ cells (green) predominantly co-label with Chx10+ nuclei (red; examples indicated by white arrows). Chx10+ neurons without (Gi)DREADD expression (examples indicated by red arrowheads) and (Gi)DREADD cells without detectable Chx10+ (examples indicated by green arrowheads) are infrequently observed. Scale bars = 20 μm.