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. 2019 Aug 5;6(4):ENEURO.0421-18.2019. doi: 10.1523/ENEURO.0421-18.2019

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Copyright © 2019 Schneider et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — Representative illustration of OPC proliferation, oligodendrocyte maturation, and differentiation in the pure OPC culture per treatment groups at 10 DIV. Co-immunostaining with DAPI (blue), anti-O4 (red), and anti-MBP (green) of coculture at 21 DIV for the untreated (UT), 0.5 μM SM (SM 0.5), 5 μM SM (SM 5), 25 μM SM (SM 25), and 0.3 μM olesoxime (OX; positive control) groups. Proliferation was measured by number of cells stained with DAPI, maturation by number of O4+ cells, and differentiation by number of MBP+ cells.