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. 2019 Jul 23;17:741–753. doi: 10.1016/j.omtn.2019.07.007

Figure 2.

Figure 2

lncRNA NR_038323 Knockdown Aggravated the HG-Reduced Expression Levels of Collagen I, Collagen IV, and Fibronectin

HK-2 cells were transfected with 50 nM lncRNA NR_038323 siRNA or scramble and then treated with or without HG for 72 h. (A) Real-time qPCR analysis of lncRNA NR_038323 expression. (B) Western blot analysis of collagen I, collagen IV, and fibronectin. (C–E) Densitometric measurement of western blot bands for collagen I (C), collagen IV (D), and fibronectin (E). Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble with HG or lncRNA NR_038323 siRNA group versus scramble group; *p < 0.05, lncRNA NR_038323 siRNA with HG group versus scramble with HG group.