Figure 6.

DUSP1 Was Identified as a Target Gene of miR-324-3p

HK-2 cells were transfected with miR-324-3p analog (100 nM) and then treated with HG for 72 h. (A) Putative miR-301a-5p complementary binding sites in the 3′ UTR of human DUSP1 mRNA. (B) The mRNA expression levels of DUSP1 were detected by RT-qPCR. (C) Western blot analysis of DUSP1 and GAPDH. (D) Densitometric analysis of proteins signals. (E) Measurement of luciferase activities after co-transfection with the 3′ UTR luciferase reporter vector of human WT or MUT-DUSP1 and miR-324-3p or miR-NC. Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble with HG group versus scramble group; *p < 0.05, miR-324-3p mimics with HG group versus scramble with HG group or DUSP1 WT/miR-324-3p mimic versus other groups.