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. 2019 Aug 25;7(16):e14219. doi: 10.14814/phy2.14219

Figure 5.

Figure 5

FF from PCOS patients with hyperandrogenism induces fibrotic injury in cultured HKC‐8 cells. (A) Representative western blots show the expression of α‐SMA and fibronectin (FN). HKC‐8 cells were stimulated with DMEM medium containing 10% of FF for 24 h. Whole‐cell lysates were analyzed by western blot. Quantitative data of α‐SMA (B) and FN (C) are shown. *P < 0.05 versus control group (n = 3). (D) Representative immunofluorescence staining show FN expression in HKC‐8 cells. HKC‐8 cells were cultured on coverslips and then stimulated with FF from PCOS patients with hyperandrogenism or controls for 24 h. The immunofluorescence of FN was detected by an antibody against FN (red) and counterstained with DAPI (blue). α‐SMA, α‐smooth muscle actin; FN, fibronectin; FF, follicular fluid; T, testosterone; PCOS, polycystic ovary syndrome; PCOS + high T, PCOS with hyperandrogenism.