FIG 1.

Spotting assay for AmB, CSP, and VOR resistance genes. (A) Spotting assay of wild-type cells growing on agar plates with the indicated concentrations of antifungal drugs. The cells were serially diluted, and a 5-μl aliquot was dropped for each spot. (B) Wild-type cells overexpressing PDR16 and PMP3, RMD9 and SWH1, or MRS3 and TRI1 were grown on plates containing a gradient of AmB, CSP, and VOR, respectively. Wild-type cells with an empty plasmid were used as controls (p-only). (C) Spotting assay for deletion mutants. The plates were incubated at 30°C for 3 days after inoculation and photographed.