TABLE 5.
MICs of artificially evolved ampC alleles
| Selection replicate | Allele | AmpC mutations | MIC in μg/ml (fold change from WT control) of: |
|||||
|---|---|---|---|---|---|---|---|---|
| Aztreonam | Ampicillin | Ceftazidime | Cefpirome | Meropenem | Imipenem | |||
| None | Wild type | 0.125 | 32 | 0.25 | 0.03125 | 0.0625 | 1 | |
| 1 | A | S15A, T16A, N49Y, R114H, Q146K, Q152R, F197Y, V239A, A256T, Q321L, N347K | 64 (512) | 256 (8) | 256 (1,024) | 0.125 (4) | 0.0625 (1) | 1 (1) |
| 1 | B | S15A, T16A, N49Y, A105T, R114H, L145R, Q146K, Q152R, V239A, A256T, Q321L, N347K | 64 (512) | 32 (1) | 8 (32) | 0.0625 (2) | 0.0625 (1) | 1 (1) |
| 2 | A | A105T, R114H, Q146K, Q152R, R159L, V239A, A256T, L320R, Q321L, N347K | 64 (512) | 128 (4) | 32 (125) | 0.03125 (1) | 0.125 (2) | 1 (1) |
| 2 | B | T21A, A105T, R114H, Q146K, R159L, A189T, V239A, A256T, L320R | 128 (1,024) | 512 (9) | 64 (256) | 0.0625 (2) | 0.0625 (1) | 1 (1) |
| 3 | A | A105T, R114H, L145R, Q146K, P180V, A256T, N347S | 64 (512) | 64 (2) | 16 (64) | 0.03125 (1) | 0.125 (2) | 1 (1) |