TABLE 4.
rdotp values and R ratios for 24 blaNDM-containing isolatesa
| Sample no. | CDC/FDA AR bank or NIH isolate |
SLGNLGDADTEHYAASAR rdotp/R value |
SLGNLGDADTEHYAASAR single-peptide call |
ASMIVMSHSAPDSR rdotp/R value |
ASMIVMSHSAPDSR single-peptide call |
AFGAAFPK rdotp/R value |
AFGAAFPK single-peptide call |
Final call | Concn (fm/μg) |
|---|---|---|---|---|---|---|---|---|---|
| L002 | CDC_148 | 1/6.87 | Positive | 0.99/6.21 | Positive | 1/3.05 | Positive | Positive | 85.9 |
| L004 | CDC_150 | 1.0/17.0 | Positive | 0.98/18.7 | Positive | 1.0/7.36 | Positive | Positive | 212.5 |
| L008 | CDC_057 | 1.0/0.58 | Positive | 0.99/0.55 | Positive | 0.98/0.35 | Positivec | Positive | 7.3 |
| L016 | CDC_152 | 1.0/8.02 | Positive | 0.98/6.72 | Positive | 1.0/3.06 | Positive | Positive | 100.3 |
| L024 | CDC_038 | 1.0/9.46 | Positive | 0.98/8.82 | Positive | 1.0/3.83 | Positive | Positive | 118.3 |
| L027 | CDC_143 | 1.0/5.25 | Positive | 0.99/3.47 | Positive | 0.99/2.01 | Positive | Positive | 65.6 |
| L030 | CDC_146 | 1.0/9.71 | Positive | 0.98/6.44 | Positive | 1.0/3.55 | Positive | Positive | 121.4 |
| L032 | CDC_151 | 1.0/51.02 | Positive | 0.99/38.15 | Positive | 1.0/19.44 | Positive | Positive | 637.8 |
| L048 | NDM-33 | 1.0/40.3 | Positive | 0.98/31.52 | Positive | 1.0/15.83 | Positive | Positive | 503.8 |
| L050 | CDC_158 | 1.0/5.58 | Positive | 0.98/3.41 | Positive | 1.0/1.94 | Positive | Positive | 69.8 |
| L051 | CDC_127 | 1.0/17.48 | Positive | 0.99/16.15 | Positive | 1.0/6.5 | Positive | Positive | 218.5 |
| L054 | CDC_162 | 1.0/48.27 | Positive | 0.99/48.01 | Positive | 1.0/19.33 | Positive | Positive | 603.4 |
| L057 | CDC_149 | 1.0/39.65 | Positive | 0.97/40.2 | Positive | 1.0/15.37 | Positive | Positive | 495.6 |
| L063 | CDC_137 | 0.95/0.01 | Negativeb | 0.98/28.08 | Positive | 1.0/12.28 | Positive | Positivee | 351.0 |
| L075 | CDC_082 | 1.0/0.28 | Positived | 0.94/0.33 | Positivec,d,e | 0.93/0.2 | Positivec ,d | Positive | 3.5 |
| L082 | CDC_145 | 1.0/5.54 | Positive | 0.98/4.21 | Positive | 0.99/2.05 | Positive | Positive | 69.3 |
| L086 | CDC_139 | 1.0/5.08 | Positive | 0.99/4.18 | Positive | 1.0/2.04 | Positive | Positive | 63.5 |
| L089 | CDC_157 | 1.0/12.3 | Positive | 0.96/9.39 | Positive | 1.0/4.94 | Positive | Positive | 153.8 |
| L094 | CDC_119 | 1.0/1.87 | Positive | 0.98/1.68 | Positive | 1.0/0.81 | positve | Positive | 23.4 |
| L098 | CDC_138 | 1.0/86.58 | Positive | 0.98/64.12 | Positive | 1.0/30.76 | Positive | Positive | 1,082.3 |
| L100 | CDC_037 | 1.0/0.11 | Positived | 0.93/0.08 | positivec,d,e | 0.94/0.11 | Possible positive | Positive | 1.4 |
| L017 | NDM-34 | 0.99/0.05 | Negative | 0.96/0.04 | Negative | 0.95/0.23c | Negative | Negative | Not detectable |
| L017g | NDM-34 | 0.94/0.04 | Negative | 0.94/0.04 | Negative | 0.93/0.10c | Negative | Negativeh | Not detectable |
| L092 | CDC_118 | 0.94/0.02 | Negativeb | 0.79/0.03 | negative | 0.85/0.05 | Negativec | Negative | Not detectable |
| L092g | CDC_118 | 1.0/17.9 | Positive | 1.0/32.5 | Positive | 1.0/7.8 | Positive | Positiveh | 223.8 |
| L099 | CDC_128 | 0.99/0.05 | Negativef | 0.99/0.05 | Negativec | 0.94/0.04 | Negativeb ,c | Negative | Not detectable |
| L099g | CDC_128 | 1.0/26.2 | Positive | 1.0/50.6 | Positive | 1.0/11.2 | Positive | Positiveh | 327.5 |
a
Three repeated tests for L017, L092, and L099 were also included in the analysis; those data were not included in the calculations of sensitivity and specificity.
b
Noise.
c
Interference.
d
Weak signal.
e
Removal of one transition.
f
Carryover.
g
A new preparation was tested after 100-sample validation was completed. The values reported represent averages of results from three replicates.
h
These data were not included in the calculation of assay sensitivity and specificity.