TABLE 1.
Results of the NG-Test Carba 5 on the collection of Pseudomonas spp. and Acinetobacter spp.a
| Category | Species | Carbapenemase | No. of isolates | NG-Test Carba 5 result for strains with: |
% sensitivity (95% CI) | % specificity (95% CI) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| NDM | IMP | VIM | OXA | KPC | ||||||
| Carbapenemase producers | ||||||||||
| KPC type | P. aeruginosa | KPC-2 | 3 | N | N | N | N | P | 100 (31.0–100) | 100 (97.2–100) |
| GES type | P. aeruginosa | GES-2 | 1 | N | N | N | N | N | ||
| P. aeruginosa | GES-5 | 1 | N | N | N | N | N | |||
| P. aeruginosa | GES-6 | 1 | N | N | N | N | N | |||
| A. baumannii | GES-14 | 1 | N | N | N | N | N | |||
| VIM type | A. xylosoxidans | VIM-1 | 1 | N | N | P | N | N | 100 (81.5–100) | 100 (96.8–100) |
| P. aeruginosa | VIM-1 | 1 | N | N | P | N | N | |||
| P. fluorescens | VIM-2 | 1 | N | N | P | N | N | |||
| P. stutzeri | VIM-2 | 1 | N | N | P | N | N | |||
| P. putida | VIM-2 | 1 | N | N | P | N | N | |||
| P. aeruginosa | VIM-2 | 9 | N | N | P | N | N | |||
| P. aeruginosa | VIM-4 | 1 | N | N | P | N | N | |||
| Acinetobacter genomosp. 16 | VIM-4 | 1 | N | N | P | N | N | |||
| A. pittii | VIM-4 | 1 | N | N | P | N | N | |||
| P. aeruginosa | VIM-6 | 1 | N | N | P | N | N | |||
| P. aeruginosa | VIM-11 | 1 | N | N | P | N | N | |||
| P. aeruginosa | VIM-28 | 1 | N | N | P | N | N | |||
| P. aeruginosa | VIM-30 | 1 | N | N | P | N | N | |||
| P. putida | VIM-60 | 1 | N | N | P | N | N | |||
| IMP type | P. stutzeri | IMP-1 | 1 | N | P | N | N | N | 62.5 (40.8–80.4) | 100 (96.8–100) |
| P. putida | IMP-1 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-1 | 1 | N | P | N | N | N | |||
| A. baumannii | IMP-1 | 1 | N | P | N | N | N | |||
| A. pittii | IMP-1 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-2 | 1 | N | P | N | N | N | |||
| A. baumannii | IMP-4 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-5 | 1 | N | P | N | N | N | |||
| A. pittii | IMP-5 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-10 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-13 | 3 | N | N | N | N | N | |||
| P. aeruginosa | IMP-15 | 1 | N | N | N | N | N | |||
| P. aeruginosa | IMP-18 | 1 | N | N | N | N | N | |||
| P. aeruginosa | IMP-19 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-26 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-29 | 1 | N | P | N | N | N | |||
| P. aeruginosa | IMP-39 | 1 | N | P | N | N | N | |||
| P. putida | IMP-63 | 1 | N | N | N | N | N | |||
| P. aeruginosa | IMP-63 | 1 | N | N | N | N | N | |||
| P. aeruginosa | IMP-71 | 1 | N | N | N | N | N | |||
| P. aeruginosa | IMP-79 | 1 | N | P | N | N | N | |||
| NDM type | P. aeruginosa | NDM-1 | 2 | P | N | N | N | N | At 15 min, 92.0 (72.4–98.6); at 30 min, 100 (83.4–100) | At 15 min, 100 (96.8–100); at 30 min, 100 (96.8–100) |
| A. baumannii | NDM-1 | 13 | P | N | N | N | N | |||
| A. baumannii | NDM-1 | 2 | Nb | N | N | N | N | |||
| A. baumannii | NDM-2 | 1 | P | N | N | N | N | |||
| A. baumannii | NDM-9 | 1 | P | N | N | N | N | |||
| OXA type | P. aeruginosa | OXA-198 | 1 | N | N | N | N | N | ||
| A. baumannii | OXA-23 | 5 | N | N | N | N | N | |||
| A. baumannii | OXA-25 | 1 | N | N | N | N | N | |||
| A. baumannii | OXA-26 | 1 | N | N | N | N | N | |||
| A. baumannii | OXA-72 | 5 | N | N | N | N | N | |||
| A. baumannii | OXA-58 | 4 | N | N | N | N | N | |||
| A. baumannii | OXA-92 | 1 | N | N | N | N | N | |||
| A. baumannii | OXA-97 | 1 | N | N | N | N | N | |||
| A. nosocomialis | OXA-420 | 1 | N | N | N | N | N | |||
| Other-carbapenemaseproducers | P. aeruginosa | GIM-1 | 2 | N | N | N | N | N | ||
| P. aeruginosa | AIM-1 | 2 | N | N | N | N | N | |||
| P. aeruginosa | SPM-1 | 1 | N | N | N | N | N | |||
| P. aeruginosa | DIM-1 | 1 | N | N | N | N | N | |||
| A. baumannii | SIM-1 | 1 | N | N | N | N | N | |||
| P. otitidis | POM-1 | 1 | N | N | N | N | N | |||
| P. alcaligenes | PAM-1 | 1 | N | N | N | N | N | |||
| Multiple-carbapenemase producers | A. baumannii | OXA-23 + NDM-1 | 6 | P | N | N | N | N | ||
| A. junii | IMP-37 + OXA-58 | 1 | N | N | N | N | N | |||
| Non-carbapenemase producers | P. aeruginosa | 50 | N | N | N | N | N | |||
| A. baumannii | 11 | N | N | N | N | N | ||||
a
P, positive; N, negative; 95% CI, 95% confidence interval, calculated using online VassarStats software (http://vassarstats.net/).
b
The NDM band was visible at 30 min but not at 15 min.