FIG 6.
Effect of DIBI and DIBI/CIP treatment on the cytokine and chemokine levels in the sera (A) and lungs (B) of BALB/c mice after i.n. challenge with A. baumannii. Groups of BALB/c mice were i.n. inoculated with 1.7 × 107 CFU of A. baumannii LAC-4. Serum and lung homogenate supernatant samples were collected and processed at 24 h, and cytokine and chemokine levels were determined using the mouse panel of 12-plex multiplex kits (Millipore) on a Luminex Magpix instrument. The data are expressed as means ± SEM of five mice. The detection limits of the assays were 2.5 to 15 pg/ml. *, P < 0.05 versus diluent vehicle-treated mice.