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T. cruzi proteasome chymotrypsin-like activity plotted as a function of time in the presence of various concentrations of suc-Leu-Leu-Val-Tyr-aminoluciferin substrate (a) or as a function of the suc-Leu-Leu-Val-Tyr-aminoluciferin substrate concentration after a 60-min biochemical reaction fitted to the Michaelis-Menten model (apparent Km = 93.5 μM [95% CI = 78.8 to 108.4 μM]) (b). Data are shown for 3 technical replicates (n = 3); the error bars represent SD.
