FIG 9.

Single-point high-throughput rescreen of 372 initial hit compounds at a concentration of 9.4 μM using the primary T. cruzi proteasome chymotrypsin-like activity assay and the secondary technology interference counterscreen assay. The data represent means of two independent replicates per assay (n = 2). The red circles represent 180 compounds that exhibited ≥30% and <45% inhibition in the primary and secondary counterscreen assays, respectively. Primary assay mean Z factor = 0.88 ± 0.04 (SD), n = 4; counterscreen assay mean Z factor = 0.79 ± 0.11 (SD), n = 4.