TABLE 1.
Biochemical assay interference mechanisms identified during the screen of the nuisance compound set using the primary T. cruzi proteasome chymotrypsin-like activity luminescence-based assay and the secondary luciferase reporter counterscreen assay
| Interference mechanism | Total no.a | No. of primary-screen hitsb | Secondary counterscreen |
|
|---|---|---|---|---|
| No. of hitsb | % detectedc | |||
| Inhibition of luminescence-coupling system | 39 | 37 | 33 | 89 |
| PDELight inhibition | 25 | 20 | 15 | 75 |
| Luciferase inhibition | 29 | 17 | 11 | 65 |
| DNA binders | 65 | 34 | 1 | 3 |
| Redox cyclers | 212 | 30 | 4 | 13 |
| Zn chelators | 28 | 5 | 1 | 20 |
| Europium donor quenchers | 26 | 2 | 0 | 0 |
| Other | 1,014 | 208 | 23 | 11 |
a
Total number of compounds in the nuisance set annotated with the listed interference mechanism (some compounds contain primary and secondary annotations).
b
Number of hits identified from nuisance set screens containing primary and/or secondary annotations for the listed interference mechanisms.
c
Percentage of hits detected by the secondary counterscreen assay relative to the primary screen assay.