TABLE 1.
Summary of resistant mutants sequenced after selection with POL7001
| Strain | PA14 no. | Gene | SNP(s) | Protein change(s) | Function | MIC (μg/ml) in MHB (fold change)a |
|||
|---|---|---|---|---|---|---|---|---|---|
| POL7001 | POL7080 | PG-1 | Colistin | ||||||
| Wild-type PA14 | 0.050 | 0.050 | 1.3 | 0.44 | |||||
| PA14-pmrBL172del | 63160 | pmrB | CGCT506C | L172delb | Two-component system | 1.6 (32) | 1.6 (32) | 43 (32) | 14 (32) |
| PA14-pmrBG188S | 63160 | pmrB | G562A | G188S | Two-component system | 0.40 (8) | 0.20 (4) | 11 (8) | 1.8 (4) |
| 21890 | hypoc | T932G | V311G | Putative oxidoreductase | |||||
| PA14-pmrBV136L | 63160 | pmrB | G406C | V136L | Two-component system | 0.80 (16) | 0.40 (8) | >43 (>32) | 3.5 (8) |
| 43080 | vgrG14 | C1325A + C1330G | A442E + H444D | Type VI secretion | |||||
| PA14-pmrBT132P | 63160 | pmrB | A394C | T132P | Two-component system | 0.40 (8) | 0.40 (8) | >43 (>32) | 3.5 (8) |
| PA14-pmrBR155H | 63160 | pmrB | G464A | R155H | Two-component system | 1.6 (32) | 1.6 (32) | >43 (>32) | 7.0 (16) |
| PA14-pmrBA330P | 63160 | pmrB | G988C | A330P | Two-component system | 0.80 (16) | 0.80 (16) | >43 (>32) | 3.5 (8) |
| PA1571-pmrBG188D | 63160 | pmrB | G563A | G188D | Two-component system | 0.20 (4) | 0.40 (8) | 10.8 (8) | >56 (>64) |
a
The fold change in MIC, relative to PA14, is shown in parentheses.
b
In-frame deletion of L172.
c
Hypothetical protein.