Figure 6.

β-Gal activity assays for the effect of two-component kinase DevS on the c-di-GMP–triggered DNA-binding affinity of DevR in M. smegmatis. A, effect of the presence or absence of DevS on the c-di-GMP–triggered activities of Ms5246p. B, effect of phosphorylation site Asp-54 of DevR on the c-di-GMP receptor activation by DevS. Left column, schematic representation of plasmids used to generate reporter strains. Right column, schematic representation of β-gal activity determined in both WT and devS-deleted M. smegmatis strains. Data are presented as Miller units (right panel). For statistical analysis, two-tailed Student's t tests were performed using GraphPad Prism 5. Null promoter lacZ, hsp60-lacZ, and Ms5247p were used as controls. Values presented are the averages of three independent biological experiments. The p values of the results (p ≤ 0.01) are indicated by **. ns, no significant difference between two groups.