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. 2019 Jul 3;294(34):12743–12753. doi: 10.1074/jbc.RA119.009644

Figure 3.

Figure 3.

SREBP-1 binds to the Hamp gene promoter. A, immunoblots of the indicated proteins and B, luciferase reporter assays with the human Hamp promoter (3 kb) for the effects of SREBP-1c in HepG2 cells. C, immunoblots of the indicated proteins and D, luciferase reporter assays with the Hamp promoter for the effects of WT or T402A mutant SREBP-1c in Hepa1–6 cells. E, locations of some regulatory elements in the Hamp promoter. F and G, luciferase reporter assays for effects of SREBP-1a on truncated Hamp promoters in Hepa1–6 cells. H, generation of point mutations in Hamp promoter fragments. I, luciferase reporter assays for effects of SREBP-1a on WT or mutant (Mu) Hamp promoters in Hepa1–6 cells. J, ChIP followed by real-time PCR analyses for binding of overexpressed HA-tagged SREBP-1a to the indicated promoters in Hepa1–6 cells. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 versus Control (vector alone or 3 kb (F), 1,000 bp (G), or WT (I) Hamp promoter, n = 3 with independent repeats).