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Knockdown of MRTF partially abrogates the α-SMA up-regulation induced by CD44 knockdown or rTGF-β1 treatment. A, α-SMA Western blotting in fibroblasts transfected with MRTF RNAi and/or CD44 RNAi, ± rTGF-β1. B, knockdown efficacy (Western blotting) of MRTF and CD44 for the experiment shown in (A). C, densitometric analyses of α-SMA Western blots using IPLab imaging software, normalized to GAPDH, pooled from four experiments; bars, mean ± range.
