Table 1.
The primers used in the characterization of G. duodenalis in the present study.
| Gene | Primer sequence (5′ – 3′) | Fragment length (bp) | Annealing temperature (°C) | Usage (s) | References |
|---|---|---|---|---|---|
| SSU rRNA | Gia2029: AAGTGTGGTGCAGACGGACTC | ~497 | 55 | Specific nested PCR of G. duodenalis | [2] |
| Gia2150c: CTGCTGCCGTCCTTGGATGT | |||||
| RH11: CATCCGGTCGATCCTGCC | ~292 | 59 | |||
| RH4: AGTCGAACCCTGATTCTCCGCCCAGG | |||||
| bg | G7: AAGCCCGACGACCTCACCCGCAGTGC | ~753 | 65 | Genotyping of G. duodenalis | [9] |
| G759: GAGGCCGCCCTGGATCTTCGAGACGAC | |||||
| Forward: GAACGAGATCGAGGTCCG | ~511 | 55 | |||
| Reverse: CTCGACGAGCTTCGTGTT | |||||
| gdh | Ghd1: TTCCGTRTYCAGTACAACTC | ~530 | 50 | Genotyping of G. duodenalis | [5] |
| Gdh2: ACCTCGTTCTGRGTGGCGCA | |||||
| Gdh3: ATGACYGAGCTYCAGAGGCACGT | 50 | ||||
| Gdh4: GTGGCGCARGGCATGATGCA | |||||
| tpi | AL3543: AAATIATGCCTGCTCGTCG) | ~605 | 50 | Genotyping of G. duodenalis | [19] |
| AL3546: CAAACCTTITCCGCAAACC) | |||||
| AL3544: CCCTTCATCGGIGGTAACTT) | ~530 | 50 | |||
| AL3545: GTGGCCACCACICCCGTGCC) |