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. 2019 Aug 23;7:e7540. doi: 10.7717/peerj.7540

Table 3. Medium composition for follicle culture experiments.

Component Catalogue number Media type
Transport media Collection media Growth media
Base media MEM with Hank’s Salts
[11575-032, Gibco]
MEM with Hank’s Salts
[11575-032, Gibco]
MEM with Earle’s Salts
[M5650, Sigma-Aldrich]
HEPES buffer 15630-080, Gibco 1%
Penicillin P7794, Sigma-Aldrich 100 IU/mL 100 IU/mL 50 IU/mL
Streptomycin S1277, Sigma-Aldrich 100 µg/mL 100 µg/mL 50 µg/mL
Ascorbic acid A61-100, Fisher 0.25 mM 0.25 mM
L-glutamine G8540, Sigma Aldrich 2 mM 2 mM
ITS+ 41400045, Thermofisher 1%
Bovine serum albumin A9418, Sigma-Aldrich 0.3% w/v 0.3% w/v