KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit α-Calbindin (1:1000) | Swant | Cat# 300; RRID:AB_10000347 |
| Rabbit α-Calretinin (1:1000) | Swant | Cat# 7699/3H; RRID:AB_10000321 |
| Sheep α-CGRP (1:1000) | Abcam | AB22560; RRID:AB_725809 |
| Rabbit α-cMaf (1:5000) | C Birchmeier, MDC, Berlin | N/A |
| Goat α-cRet (1:250) | R&D Systems | Cat# AF482; RRID:AB_2301030 |
| Goat α-CTb (1:4000), | List Laboratories | Cat# 703; RRID:AB_10013220 |
| Sheep α-Digoxigenin-AP, Fab fragments (1:2000) | Roche | Cat# 11093274910; RRID:AB_514497 |
| Rabbit α-DsRed (1:1000) | Clontech | Cat# 632496; RRID: AB_10013483 |
| Mouse α-Gephyrin (1:8000) | Synaptic Systems | Cat# 147 011; RRID:AB_887717 |
| Rabbit α-GFAP (1:500) | Dako | Cat# Z0334; RRID:AB_10013382 |
| Chicken α-GFP (1:000) | Aves | Cat # GFP-1020; RRID:AB_10000240 |
| Goat α-GFP (1:1000) | Abcam | Cat# ab6673; RRID:AB_305643 |
| Rabbit α-GRPR (1:100) | MBL | Cat# LS-A831; RRID:AB_591750 |
| Rabbit α-GRPR (1:1000) | Abcam | Cat# ab39883; RRID:AB_880315 |
| Guinea pig α-Lmx1b (1:1000) | M Goulding | N/A |
| Rabbit α-MafA (1:5000) | C Birchmeier, MDC, Berlin | N/A |
| Mouse α-NeuN (1:1000) | Millipore | Cat# MAB377; RRID:AB_2298772 |
| Rabbit α-Neuropeptide Y1 Receptor (1:100) | Alomone Labs | Cat# ANR-021: RRID:AB_2040030 |
| Rabbit α-NF200 (1:1000) | Sigma | Cat# N4142; RRID:AB_477272 |
| Rabbit α-NK1R (1:500) | Advanced Targeting Systems | Cat# AB-N33ap; RRID:AB_458739 |
| Rabbit α-NPY (1:1000) | Peninsula Lab | Cat# T-4070.0050; RRID:AB_518504 |
| Rabbit α-Pax2 (1:200) | Zymed | Cat# 71–6000; RRID:AB_2533990 |
| Rabbit α-Parvalbumin (1:2000) | Swant | Cat# PV-25; RRID:AB_10000344 |
| Rabbit α-PKCγ (1:1000) | Santa Cruz | Cat# SC-211; RRID:AB_632234 |
| Rat α-RFP (1:1000) | Chromotek | Cat#5F8; RRID: AB_2336064 |
| Goat α-RORα (1:100) | Santa Cruz | Cat# sc-6062; RRID:AB_655755 |
| Rabbit α-S100β (1:500) | Dako | Cat# Z0311; RRID:AB_10013383 |
| Chicken α-TrkB (1:1000) | R&D Systems | Cat# AF1494; RRID:AB_2155264 |
| Goat α-TrkC (1:1000) | R&D Systems | Cat# AF1404; RRID:AB_2155412 |
| Guinea pig α-VGAT (1:1000) | Synaptic Systems | Cat# 131004; RRID:AB_887873 |
| Guinea pig α-vGluT1 (1:1000) | Millipore | Cat# AB5905; RRID: AB_2301751 |
| Bacterial and Virus Strains | ||
| EnvA-pseudotyped, ∆G-mCherry | Janelia Viral Core/HHMI | Wickersham et al., 2007 |
| EnvA-pseudotyped, ∆G-dsRed-Express | Janelia Viral Core/HHMI | Wickersham et al., 2007 |
| AAV2/1-hSyn-DIO-SypHTom | Koch et al., 2017 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor 647 Conjugate (1:500) | Invitrogen | Cat# I32450; RRID:SCR_014365 |
| Cholera Toxin Subunit b (Recombinant), Alexa Fluor 647 Conjugate | Invitrogen | Cat# C34778 |
| Diphtheria Toxin, Unnicked, from Corynebacterium diphtheria | List Laboratories | Cat# 150 |
| Clozapine N-oxide | Sigma | Cat# C0832 |
| Blank saporin | Advanced Targeting Systems | Cat# IT-21 |
| Bombesin-saporin | Advanced Targeting Systems | Cat# IT-40 |
| [Sar9, Met(O2)11]-substance P-saporin | Advanced Targeting Systems | Cat# IT-11 |
| Neuropeptide Y (human, rat) | Tocris | Cat# 1153 |
| [Leu31, Pro34]-NPY (100 µg kg−1) | M Goulding | N/A |
| BIBP 3226 trifluoroacetate | Tocris | Cat# 2707/1 |
| BMS 193885 | Tocris | Cat# 3242/10 |
| Chloroquine diphosphate | Sigma | Cat# C6628 |
| Histamine | Sigma | Cat# H7125 |
| Compound 48/40 | Sigma | Cat# C2313 |
| SLIGRL-NH2 | Abcam | Cat# ab120176 |
| Capsaicin | Sigma | Cat# M2028 |
| Formalin | Sigma | Cat# HT501128 |
| Kynurenic acid | Sigma | Cat# K3375 |
| Picrotoxin | Sigma | Cat# P1675 |
| Strychnine | Sigma | Cat# S0532 |
| NBT | Roche | Cat# 11383213001 |
| BCIP | Roche | Cat# 11383221001 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Y1Cre | The Jackson Laboratory | JAX stock #030544; RRID:IMSR_JAX:030544 |
| Mouse: FLPo-10 | The Jackson Laboratory | JAX stock #011065; RRID:IMSR_JAX:011065 |
| Mouse: Y1::eGFP | Gene Expression Nervous System Atlas (GENSAT) | RRID:MMRRC_010554-UCD |
| Mouse: NPY::Cre | Bourane et al., 2015a | N/A |
| Mouse: SstCre | The Jackson Laboratory | JAX stock #013044; RRID:IMSR_JAX:013044 |
| Mouse: SstFlpO | The Jackson Laboratory | JAX stock #028579; RRID:IMSR_JAX:028579 |
| Mouse: Lbx1Cre | Sieber et al., 2007 | MGI: 104867 |
| Mouse: Lbx1FlpO | Bourane et al., 2015a | N/A |
| Mouse: Ai14lsl-Tom | The Jackson Laboratory | JAX stock #007908; RRID:IMSR_JAX:007908 |
| Mouse: Ai65ds-Tom | The Jackson Laboratory | JAX stock #021875; RRID:IMSR_JAX:021875 |
| Mouse: R26ds-hM3D | The Jackson Laboratory | JAX stock #026942; RRID:IMSR_JAX:026942 |
| Mouse: R26ds-hM4D | Bourane et al., 2015a | N/A |
| Mouse: R26ds-HTB | Bourane et al., 2015b | N/A |
| Mouse: R26ds-ReaChR | Hooks et al., 2015 | JAX stock #024846; RRID:IMSR_JAX:024846 |
| Mouse: R26lsl-TVA | Seidler et al., 2008 | MGI:3814188 |
| Mouse: NPY KO | Karl et al., 2008 | N/A |
| Mouse: Y1f/f | Bertocchi et al., 2011 | N/A |
| Mouse: GRP::eGFP | MMRRC | RRID:MMRRC_010444-UCD |
| Software and Algorithms | ||
| Adobe Illustrator and Photoshop CS5 | Adobe | https://www.adobe.com |
| Prism 5 | GraphPad | https://www.graphpad.com |
| Excel 365 | Microsoft | https://www.microsoft.com/en-us/ |
| ImageJ Cell Counter Plugin | Kurt de Vos, Univ. of Sheffield, UK | https://imagej.nih.gov/ij |
| pClamp10.4 and Clampfit | Molecular Devices | https://www.moleculardevices.com |