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. 2019 Aug 26;2(5):e201900431. doi: 10.26508/lsa.201900431

Figure 6. Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling.

Figure 6.

Protein from BxPC-3, PANC-1, MiaPaCa-2, and MOH parental, single and double ORP knockdowns as well as cells transfected with empty vector control (pLKO.1) were harvested, and 20 μg was subjected to SDS–PAGE and used for Western blotting. EGFR, MAPK, and PI3K signaling were assayed as pEGFR, ppERK, and pAKT levels, respectively. Amplification of YAP-1 was also evaluated. Total ERK, total AKT, and β-actin levels were used as loading controls.