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. 2018 Jun 5;103(5):610–616. doi: 10.1136/bjophthalmol-2018-311849

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Representation of the algorithm used to process the images. In order to isolate the red emission fluorescence component (REFC) and obtain the 450-red filtered (RF) images, the colour fundus autofluorescence (FAF) image was duplicated and the two resulted images underwent the following process: (1) one image was first transformed in a 32-bit image and thus converted in an 8-bit greyscale image; (2) the other (duplicate) image subject to the ‘split channels’ function, which splits the image into the respective red and green image channels (note: no blue channel is present in a colour FAF image). Finally, in order to optimise the visualisation of those regions where the REFC is absent or the autofluorescence is mainly due to the presence of green emission fluorescence component, a ‘subtraction’ function was performed, subtracting the ‘green channel’ of the colour FAF image from the obtained greyscale FAF image, to yield the 450-RF FAF image.