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. 2019 Aug 8;13:180. doi: 10.3389/fnbeh.2019.00180

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Copyright © 2019 Haghani, Karia, Cheng and Mathuru.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Longer duration improves measures of acclimation. The mean difference between the first 2 (0–2) and the last 2-min (16–18) are shown in Gardner-Altman estimation plots. The percentage of total time in the (A) bottom third and (B) bottom half, the frequency of (C) immobility episodes and (D) erratic swimming episodes are reduced in the last 2-min. Fish in this repetition show more (E) immobility episodes and fewer (F) erratic swimming episodes in the first 2-min compared to the fish in the first experiment shown in Figure 1. Each dot in the group represents the response of one individual. N = 20 per group. Groups are plotted on the left axis, while the mean difference between the groups is depicted as a black dot and is plotted on a floating axis on the right. Ends of the bar around the mean shows 95% confidence interval. The shaded region in gray is 5,000 bootstrap sampling distribution.