Figure 3.

Expression of CFTR, phospho-FAK Tyr861, and phospho-paxillin in HaCaT cells after treatment with ICG-PDT-conditioned medium. (A) Immunofluorescence images of HaCaT cells treated with ICG-PDT-conditioned medium (5 J/cm² and 100 μg/mL ICG) at different time points. The cells were fixed and stained with the primary antibodies anti-CFTR (green) and phospho-FAK Tyr861 (p-FAK; red), and DAPI (blue). The figures show representative images from three separated experiments. (B) Representative immunoblots from three separate experiments revealed dynamic expressions of CFTR, phospho-FAK Tyr861 (p-FAK), total FAK (t-FAK), phospho-paxillin Tyr 118 (p-paxillin), and β-actin in HaCaT cells treated with the same conditioned medium. (C) The protein expression levels were quantified with ImageJ software (*p < 0.05, two-way ANOVA, Bonferroni comparison test). Scale bar = 10 μm. Figures show representative data from three separate experiments. DAPI, 4′,6-diamidino-2-phenylindole.