Figure 1.

Removal of miR-iab4/iab8 Disrupts Larval and Adult Self-Righting Behavior

(A) Drosophila melanogaster life cycle.

(B) Diagram of SR behavioral response in larvae (top) and adults (bottom).

(C) Quantification of the time required for the successful completion of the SR behavior along larval stages and in the adult (mean ± SEM; N = 63–70 larvae for L1, 27–28 for L2, and 25 for L3, and N = 49–54 adult flies) in wild-type controls (w¹¹¹⁸, gray) and miR-iab4/iab8 mutants (ΔmiR, red). Analysis of SR behavior throughout development shows that ΔmiR mutants have defects across larval stages and in the adult. A nonparametric Mann-Whitney U test was performed to compare treatments; ^∗∗∗p < 0.001. AEL, after egg laying. (NB: Experiments in adult flies were conducted on wingless specimens, but similar results were obtained using different anesthesia methodologies; STAR Methods.)

See also Figures S1 and S3 and Videos S1 and S2.