Figure 2.

Effects of miRNA Regulation on Leg Movement

(A) Description of self-righting (SR) movement in wild-type (top, WT) and miRNA mutant (bottom, ΔmiR) adult flies. Manual video analysis shows that SR behavior in adult WT flies involves several components, including (1) detection of abnormal (upside-down) body orientation, (2) horizontal stirring of legs and body, (3) attempts to grab substrate, (4) coordinated movement of left and right third legs anteriorly until substrate is grabbed, (5) lifting of body by the third legs, (6) tilt of the whole body forward, and (7) return to normal position. (NB: Experiments in adult flies were conducted on wingless specimens; see STAR Methods and Figure 1 legend.) See also Videos S1 and S2.

(B–G) Quantification of leg movement levels in WT control (w¹¹¹⁸) and ΔmiR flies. Schematic of the paradigm and the regions of interest (black rectangles) drawn to quantify leg movements (STAR Methods) (B). Average heatmaps of leg movements (C) and their corresponding contours (D), and range of movements as defined by the movement contours, in azymuth (right) and elevation (left) axis (E) in ΔmiR flies compared to WT (mean ± SD; two series of experiments, each with N = 10–12 individuals). Color code indicates amplitude of movement, with warm colors representing high levels. Quantification of the leg movements in the ROIs as a function of time (F and G) (mean ± SEM; two independent series of experiments, each with N = 10–12 individuals).

Non-parametric Mann-Whitney U test was performed to compare groups; ^∗∗∗p < 0.001 and ^∗∗∗∗p < 0.001. A.U., arbitrary units. See also Figures S2 and S4.