Figure 6.

Conditional Increase of Ubx Expression after Development Is Completed Is Sufficient to Alter Adult Behavior

(A) Conditional expression experiment in which Ubx protein is upregulated only once development has been completed. Graphic representation of Gal4 and Gal80 activities over developmental time. NB: At 18°C, Gal80^ts represses Gal4 activity; at 31°C, the Gal80^ts role is inactivated, allowing for VT006878-Gal4-mediated induction of Ubx (green) in ventral lin15 motor neurons. Maximal induction is achieved approximately 4 days after eclosion (Ti).

(B) SR behavior test (STAR Methods) performed at Ti reveals that post-developmental induction of Ubx in VT006878 neurons Tub-Gal80^ts; VT006878 > Ubx (w; UAS-Ubx/+; Tub-Gal80^ts,+/ +, VT006878-Gal4) (blue) is sufficient to cause SR defects in comparison to control line Tub-Gal80^ts;VT006878 > Nls::GFP (w; UAS- Nls::GFP /+; Tub-Gal80^ts,+/ +, VT006878-Gal4) (mean ± SEM; N = 19–25 flies). A non-parametric Mann-Whitney U test was performed to compare treatments; ^∗∗∗p < 0.001.

(C and D) Control treatment for the conditional expression of Ubx in adult Drosophila. Graphic representation of Gal4 and Gal80 activities over developmental time (C). At 18°C, Gal80^ts represses Gal4 activity, thus blocking VT006878-Gal4-mediated induction of Ubx in VT006878/ventral lin15 neurons. Under Gal80-mediated repression, there is no induction of Ubx expression in VT006878 cells and no statistically significant changes in SR times are observed when comparing the experimental line Tub-Gal80^ts;VT006878; > Ubx (blue) with the control line VT006878;Tub-Gal80^ts>Nls::GFP (gray) (mean ± SEM; N = 19–25 flies) (D). A non-parametric Mann-Whitney U test was performed to compare treatments; ^∗∗∗p < 0.001. (NB: Experiments in adult flies were conducted on wingless specimens; see STAR Methods and Figure 1 legend.)