Table 1. Primer sequences for RT-qPCR.
Genes | Primer sequences (5'-3') |
miR-199a | F: 5'-ACACTCCAGCTGGGTCCCTGAGACCCTTTA-3' |
R :5'-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAG-3' | |
AGAP2 | F: 5'-GCAGCTACTATGAGACTTGTGC-3' |
R: 5'-GTGACCAACATTCGGTGAGGA-3' | |
U6 | F: 5'-CGCTTCGGCAGCACATATACTA-3' |
R: 5'-CGCTTCACGAATTTGCGTGTCA-3' | |
GAPDH | F: 5'-AGAAGGCTGGGGCTCATTTG-3' |
R: 5'-AGGGGCCATCCACAGTCTTC-3' |
Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; F, forward; R, reverse; miR-199a, microRNA-199a; AGAP2, Arf GTPase-activating protein-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.