Skip to main content
. 2019 Aug 5;11(15):5300–5318. doi: 10.18632/aging.102092

Table 1. Primer sequences for RT-qPCR.

Genes Primer sequences (5'-3')
miR-199a F: 5'-ACACTCCAGCTGGGTCCCTGAGACCCTTTA-3'
R :5'-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAG-3'
AGAP2 F: 5'-GCAGCTACTATGAGACTTGTGC-3'
R: 5'-GTGACCAACATTCGGTGAGGA-3'
U6 F: 5'-CGCTTCGGCAGCACATATACTA-3'
R: 5'-CGCTTCACGAATTTGCGTGTCA-3'
GAPDH F: 5'-AGAAGGCTGGGGCTCATTTG-3'
R: 5'-AGGGGCCATCCACAGTCTTC-3'

Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; F, forward; R, reverse; miR-199a, microRNA-199a; AGAP2, Arf GTPase-activating protein-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.