Table 3. Descriptive statistics and comparisons of standard semen parameters and DFI between men aged <40 y and ≥40 y with abnormal semen parameters.
| Parameters n median (range) mean±SD |
< 40 y (n = 535) |
≥ 40 y (n = 141) |
p |
| Age (y) | 535 32.00 (18.00–39.00) 31.72 (4.83) |
141 44.00 (40.00–70.00) 46.24 (6.88) |
0.00001 |
| Semen volume (mL) | 535 3.00 (0.50–10.00) 3.32 (1.55) |
141 3.00 (0.15–10.00) 2.99 (1.60) |
0.01952 |
| Sperm concentration (×106/mL) | 535 22.50 (0.001–238.30) 32.62 (32.71) |
141 22.30 (0.10–210.00) 38.61 (42.14) |
0.55474 |
| Total number of spermatozooa (x106) | 535 62.50 (0.002–800.00) 107.74 (124.64) |
141 59.50 (0.15–689.00) 104.82 (125.21) |
0.44229 |
| Morphologically normal spermatozoa (%) | 535 2.00 (0.00–22.00) 3.04 (3.58) |
141 2.00 (0.00–11.00) 2.48 (2.61) |
0.24004 |
| TZI | 495 1.72 (1.02–2.62) 1.73 (0.24) |
139 1.68 (1.26–2.33) 1.70 (0.21) |
0.16066 |
| Progressive motility (%) | 535 27.00 (0.00–84.00) 30.34 (18.53) |
141 30.00 (0.00–88.00) 31.65 (20.47) |
0.59229 |
| Non-progressive motility (%) | 535 15.00 (0.00–59.00) 17.14 (10.79) |
141 11.00 (0.00–54.00) 13.79 (9.70) |
0.00033 |
| Immotile sperms (%) | 535 50.00 (6.00–100.00) 52.38 (19.09) |
141 52.00 (4.00–99.00) 54.55 (22.99) |
0.21761 |
| Eosin-negative spermatozoa – live cells (%) | 531 64.00 (0.00–98.00) 59.66 (19.19) |
139 58.00 (2.00–97.00) 57.44 (21.21) |
0.23521 |
| Hos-test-positive spermatozoa – live cells (%) | 379 57.00 (0.00–90.00) 53.84 (19.21) |
117 57.00 (11.00–90.00) 54.47 (19.09) |
0.84956 |
|
Round cells concentration (x 106/mL) |
511 0.20 (0.00–14.68) 0.68 (1.48) |
137 0.10 (0.00–7.03) 0.46 (1.00) |
0.10633 |
| Peroxidase-positive cells (x106/mL) | 515 0.00 (0.00–11.70) 0.43 (1.17) |
137 0.00 (0.00–4.80) 0.30 (0.74) |
0.21906 |
| DFI (%) | 132 10.54 (1.00–60.20) 13.92 (10.99) |
44 20.45 (0.76–75.94) 21.85 (15.18) |
0.00120 |
DFI – sperm DNA fragmentation index, n – number of subjects, SD – standard deviation, TZI – teratozoospermia index, p – significance of differences between compared groups, Mann–Whitney U test