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. Author manuscript; available in PMC: 2019 Aug 27.
Published in final edited form as: Adv Exp Med Biol. 2019;1115:167–190. doi: 10.1007/978-3-030-04278-3_8

Figure 4: bSUMs for preferred directional insertion of ion channels in CHOL-rich membranes.

Figure 4:

Chemical functionalization added ligand to the surface of silica beads (A). Channels bind to the ligands and orient themselves (B). Addition of detergent-solubilized lipids and slow removal of the detergents by BioBeads leads to the formation of one bilayer membranes around each bead (D), which is the bSUM. The channels in bSUMs can be patched using a planar glass electron for electrical recordings (E). Adapted from ref [3].