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. 2019 Aug 26;9:12354. doi: 10.1038/s41598-019-48527-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of vaginal wall injury on FBLN5. Nulliparous young rats were either not injured or injured as described in Materials and Methods. Injured rats were treated with hydrogel (HG) alone or HG + FBLN5 (10 µg/ml) (n = 5 in each group). Data represent mean ± SEM. After 48 h, vaginal muscularis was harvested and analyzed for levels of endogenous or recombinant FBLN5. (A) Upper blot was incubated with anti-FBLN5 whereas the lower blot was incubated with anti-His tag antibody to recognize recombinant FBLN5. (B) Quantitative analysis of endogenous FBLN5 in non-injured and injured vagina treated with HG or HG + FBLN5. *P < 0.05 compared with control (C) Immunoblot analysis of urea extracts of muscularis from control uninjured rats before and 12, 24, 48 h and 7D after cut injury. The 26 kDa bands represent nonspecific binding to IgG. (D) Quantitative analysis of FBLN5 levels in control uninjured (Ctl) or injured animals as a function of time after cut (open symbols) or wedge injury (solid squares). Data represent mean ± SEM. Uninjured control rats (n = 6) are presented with 20 cut and 12 wedge injuries divided equally among the four time points At 7 days after injury, horizontal line represents mean, individual values are presented. Data represent mean ± SEM, ^*p < 0.05 compared with control; ^**p < 0.05 compared with wedge.