Fig. 2.

Validation of PBA using STV-biotin-oligonucleotide complexes. Tetrameric streptavidin molecules were incubated with four different biotinylated oligonucleotides, either separately or in a mixture of all four oligonucleotides. Streptavidin and biotinylated oligonucleotides were combined at molar ratios of 1:10 or 10:1. a The numbers of observed complexes with different numbers of proteinTags and moleculeTags are summarized in pie charts. b When the oligonucleotides were in excess, the numbers of complexes from separate or mixed oligonucleotides incubation with 2, 3, or 4 moleculeTags were grouped according to the number of proteinTags per complex. Theoretical ratios were calculated for the mixed sample