Fig. 5.

Quantification of exosomes from different sources according to their surface protein combinations, as revealed by PBA. a Protein combinations selective for either K562 exosomes (orange) or prostasomes (blue), compared to exosomes from serum (gray), were sorted based on the number of exosomes with specific combinations. Here, the top 50 for each exosome are displayed. b, c Serial dilution of K562 exosomes or prostasomes spiked in serum exosomes were quantified using K562- or prostasomes-selective combinations, respectively. The exosomes identified with either K562- or prostasome-selective combinations are indicated by orange and blue filled circles in the tSNE plots in b, and with orange and blue bars, respectively, in the bar plots in c. The sizes of the colored circles in (b) are proportional to the number of exosomes with the same protein combination. Each two vertically adjacent tSNE plots and two horizontally adjacent bars are illustrating two replicates