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. 2019 Aug 26;9:12359. doi: 10.1038/s41598-019-48821-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analyses. (A) Western blot analyses for purified fucosylated AFP, afucosylated AFP, and ALP. Lane 1: fucosylated AFP, Lane 2: afucosylated AFP, Lane 3: ALP. (B) Western blot analyses for AFP purified from conditioned media of wild-type and FUT8-deficient HepG2 cells. Lane 1: AFP from wild-type HepG2 cells, Lane 2: AFP from FUT8-deficient HepG2 cells. (C) Western blot and AAL blot analyses for conditioned media of wild-type and FUT8-deficient HepG2 cells. Lane 1: Supernatant of wild-type HepG2 cells, Lane 2: Supernatant of FUT8-deficient HepG2 cells. (D) Western blot analyses for AFP following co-precipitation with an anti-AFP polyclonal antibody from human serum. Lane 1: sample 1, Lane 2: sample 2, Lane 3: sample 3. All images are detected individually, and cropped from different blots. Full-length blots of (A–D) are presented in Supplementary Figs 1–4, respectively.