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. 2019 Aug 26;9:12370. doi: 10.1038/s41598-019-48876-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The matrix effect of various matrices on the peak area of the deuterated internal standards. Matrix effect was determined by the ratio of the peak area of the deuterated internal standards in a matrix sample to the peak area of the deuterated internal standards in a water sample. Physiological PC, salt, a combination of both and plasma have been evaluated for both FIA-MS and LC-MS.