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. 2019 Aug 26;9:12370. doi: 10.1038/s41598-019-48876-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat map of the log2 transformed serum level differences between the fasted and non-fasted time point. Blue and red cells represent increased and decreased SLM-challenged metabolite levels in serum, respectively. The volunteers and metabolites are sorted by hierarchical clustering. Dendograms are used to show distances between samples (rows) and metabolites (columns). Based on the hierarchical clustering, three groups could be identified indicating volunteers with increased levels (A), limited differences (B) and decreased levels (C). It can be clearly seen that A and C consist of mainly male and female volunteers, respectively.