Table 1.

Method validation parameters for the fast LC-ESI-MS/MS method.

Analyte	Elution time (min)	LOD (µM)	LLOQ (µM)	Linearity (R2)	Blank effect (%)	Repeatability (RSD, %)	Intermediate precision (RSD, %)
TMAO	0.33	0.04	0.1	0.999	0.5	6.1	5.2
Betaine	0.35	0.9	2.7	0.999	0.8	1.2	3.4
Choline	0.33	0.5	1.5	0.999	0.7	3.6	4.2
Carnitine	0.33	0.7	1.7	0.999	0.5	6.2	6.7
GBB	0.33	0.02	0.04	0.998	1.3	5.9	6.3