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. 2019 Aug 26;9:12353. doi: 10.1038/s41598-019-48787-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Single-cell resolution of TREM2, SNAP25 and DKK1 mRNAs present differential cell-type expression. Confocal images of control hippocampal slices showing TREM2, SNAP25 and DKK1 co-localising with different cell-type-specific markers including SLC1A2 (astrocytic), MAP2 (neuronal) and P2RY12 (microglial). (A–C) RNAscope of TREM2 (red) showing no colocalization with SLC1A2 (A-A’; green) nor MAP2 (B-B’; green), but P2RY12 (C-C’; green). (D–F) In situ hybridisation of SNAP25 (red) showing no colocalization with SLC1A2 (D-D’; green) or P2RY12 (F-F’; green), but MAP2 (E-E’, green) (G–I) RNAscope of DKK1 (red) showing colocalization with SLC1A2 (G-G’; green) and MAP2 (H-H’, green) but not with P2RY12 (I-I’; green). DAPI indicates nuclear staining (blue) in all cases. Scale bars 50 µm, zoom imaged 20 µm.